DIVERSIFIED APPLICATIONS OF POLYHYDROXYALKANOATES

Prabhat Kumar Singh, A. K. Sen Sen, A. S. Vidyarthi

Abstract


Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible polyesters which are accumulated intracellularly to levels as high as 90% of the cell dry weight (CDW) under conditions of nutrient stress by numerous Gram positive bacteria, Gram negative bacteria as well as some archaebacteria. They are multitalented biopolymers with suitability of diverse applications. The primary applications of PHAs were as substitute for replacements of petrochemical based polymers to be used in packaging and coating applications. Nowadays the rising interests of different research groups in PHA the number of applications of PHA’s became extremely expanded. This review emphasizes the established and emerging applications of PHAs in industrial field, medical application such as in tissue engineering, drug delivery, agricultural applications, applications as biofuel, applications in protein purification and other enzyme purification. Today several companies are involved production of PHA in large scale as well as PHA related research and development activities.

Keywords


PHA, Biomaterial, Industrial Applications, Tissue Engineering, Biopolymer market

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References


Ahmed, T., Marcal, H., Lawless, M., Wanandy, N.S., Chiu, A., and Foster, L.J.R., (2010) Polyhydroxybutyrate and its Copolymer with Polyhydroxyvalerate as Biomaterials: Influence on Progression of Stem Cell Cycle. Biomacromolecules, 11, p. 2707-2715,

Aebischer, P., (1990) Piezolectric nerve guidance channels. EP 0307437 A4.

Akhtar, S., Pouton, C.W., and Notarianni, L.J., (1992) Crystallization behaviour and drug release from bacterial polyhydroxyalkanoates. Polymer Bulletin, 33, p. 117-126.

Bian, Y.Z., Wang, Y., Guli, S., Chen, G.Q., and Wu, Q., (2009) Evaluation of poly(3- hydroxybutyrate-co-3-hydroxyhexanoate) conduits for peripheral nerve regeneration,” Biomaterials, 30, p. 217-225.

Bissery, M. C., Valeriote, F., and Thies, C., (1984) In vitro and in vivo evaluations of CCNU-loaded microspheres prepared from polylactide and poly (β-hydroxybutyrate)” in microspheres and drug therapy, Pharmaceutical, Immunological and Medical Apsects, S. S. Davis, et al., Eds, p. 217-227, Elsevier, Amsterdam.

Banki, M.R., Gerngross, T.U., and Wood, D.W., (2005) Novel and economical purification of recombinant proteins: Intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association,” Protein Science, 14, p. 1387-1395

Bond, E.B., and Noda, I., (2006) Polyhydroxyalkanoate copolymer/starch compositions for laminates and films. US Patent 7077994.

Bourbonnais, R., and Marchessault, R. H. (2010) Application of polyhydroxyalkanoate granules for sizing of paper. Biomacromolecules, 11(4), pp. 989-993.

Brigham, C.J., and Sinskey, A.J. (2012) Applications of polyhydroxyalkanoates in the medical industry. International Journal of Biotechnology for Wellness Industries, 1, pp. 53-60.

Burke, T.R., Knight, M., and Chandrasekhar, B., (1989) Solid-phase synthesis of viscosin, a cyclic depsipeptide with antibacterial and antiviral properties. Tetrahedron Letters, 30, pp. 519-522.

Castilho L.R., Mitchell D.A. and Freire D.M.G. (2009) Production of polyhydroxyalkanoates (PHAs) from waste materials and by-products by submerged a solid-state fermentation. Bioresource Technology; 100, p.5996–6009.

Chen, G.Q.. and Wu, Q., (2005) The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 26, 6565-6578.

Chen, W., and Tong, Y.W., (2012) PHBV microspheres as neural tissue engineering scaffold support neuronal cell growth and axon-dendrite polarization,” Acta Biomaterialia, 8(2), p. 540-548.

Cheng, S., Wu, Q., Yang, F., Xu, M., Leski, M., and Chen, G.Q., (2005) Influence of DL-beta hydroxybutyric acid on cell proliferation and calcium influx. Biomacromolecules. 6, pp. 593-597.

Defoirdt, T., Sorgeloos, P., and Bossier, P., (2011) Alternatives to antibiotics for the control of bacterial disease in aquaculture,” Current Opinion in Microbiology, 14(3), pp. 25-258.

de Vlieger, J.J., (2003) Green plastics for food packaging,” in Novel food packaging techniques, R. Ahvenainen Ed., pp. 519-534, Wood head Publishing in Food Science and Technology, Netherland.

Doyle, C., Tanner, E.T., and Bonfield, W., (1991) In vitro and in vivo evaluation of polyhydroxybutyrate and of polyhydroxybutyrate reinforced with hydroxyapatite. Biomaterials, 12, p. 841-847.

Evans, G.R.D., and. Persing, J.A., (2007) Soft-Tissue Surgery of the Craniofacial Region. Taylor & Francis Group.

Fuller, T.J., Marchessault, R.H., and Bluhm, T.L., (1991) Toner and developer compositions containing biodegradable semicrystalline polysters. US patent 5004664.

Galego, N., Rosza, C., Sanchez, R., Fung, J., Vazquez, A., and Tomas, J.S., (2000) Characterization and application of poly (β hydroxyalkanoates) family as composite biomaterials. Polymer Testing. 19, p. 485-492.

García-García, J.M., Garrido, L., Quijada-Garrido, I., Kaschta, J., Schubert, D.W., and. Boccaccini, A.R., “Novel polyhydroxyalkanoates based composites containing Bioglass® and calcium sulfate for bone tissue engineering. Biomedical Materials, 7(5), doi:10.1088/1748-6041/7/5/0541052012.

Gilbertson, W.G., Gray, F.B., Mcavoy, C.D., Melik, H.D., Noda, I., and Zhao, J.J., (2008) Molded articles comprising polyhydroxyalkanoate copolymer and an environmentally degradable thermoplastic polymer. The Procter & Gamble Company, EP1620507B.

Gould, P.L., Holland, S.J., and Tighe, B.J., (1987) Polymers for biodegradable medical devices. 4-Hydroxybutyrate valerate copolymers as nondisintegrating matrices for controlled-releaseoral dosage forms. International Journal of Pharmaceutics, 38, p. 231-237.

Grage, K., Jahns, A.C., Parlane, N., Palanisamy, R., Rasiah, I.A., Atwood, J.A., and Rehm, B.H.A., (2009) Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications,” Biomacromolecules,10, p. 660-669.

Gross R.A. and Kalra, B. (2002) Biodegradable polymers for the environment. Science; 297, p.803–807.

Iovine, C.P., and Walton, J.H., (1994) Poly(hydroxybutyrate/hydroxyvalerate) copolymers for fiber bonding. US Patent 5656367.

Hablot, E., Bordes, P., Pollet, E., and Avérous, L., (2008) Thermal and thermo mechanical degradation of PHB-based multiphase systems. Polymer Degradation and Stability, 93, 413-421.

Halet, D., Defoirdt, T., Van Damme, P., Vervaeren, H., Forrez , I., Van de Wiele, T., Boon, N., Sorgeloos, P., Bossier, P., and Verstraete, W., (2007) Poly-beta-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS Microbiology Ecology, 60, pp. 363-369.

Huang, W., Shi, X., Ren, L., Du, C., and Wang, Y., (2010) PHBV microspheres – PLGA matrix composite scaffold for bone tissue engineering. Biomaterials, 31, pp. 4278-4285

Holme, P.A., (1985) Application of PHB: a microbially produced biodegradable thermoplastic. Physics in Technology, 16, p. 32-36.

Ihssen, J., Magnani, D., Thöny-Meyer, L., and Ren, Q., (2009 ) Use of Extracellular Medium Chain Length Polyhydroxyalkanoate Depolymerase for Targeted Binding of Proteins to Artificial Poly[(3-hydroxyoctanoate)-co-(3-hydroxyhexanoate)] Granules,” Biomacromolecules ,10, p. 1854-1864.

Jones, R.D., Price, J.C., and Bowen, J.M., (1994) In vitro and in vivo release of metoclopramide from a subdermal diffusion matrix with potential in preventing fescue toxicosis in cattle. Journal of Controlled Release, 30, p. 35-44.

Kadouri, D., Jurkevitch, E. ,and Okon, Y., (2003) Involvement of the reserve material poly-beta-hydroxybutyrate (PHB) in Azospirillim brasilense in stress endurance and root colonization. Applied and Environmental Microbiology, 69, p. 3244-3250,

Kadouri, D., Jurkevitch, E., and Okon, Y., (2005) Ecological and agricultural significance of bacterial polyhydroxyalkanoates. Critical Reviews in Microbiology, 31, p. 55-67.

Kashiwaya, Y., Takeshima, T., Mori, N., Nakashima, K., Clarke, K., and Veech, R.L., (2000) D-β-Hydroxybutyrate protects neurons in models of Alzheimer’s and Parkinson’s disease. Proceedings of the National Academy of Sciences USA, 97, pp. 5440-5444.

Katayama, M., Hiraide, A., Sugimoto, H., Yoshioka, T., and Sugimoto, T., (1994) Effect of ketone bodies on hyperglycemia and lactic acidemia in hemorrhagic stress,” Journal of Parenteral and Enteral Nutrition, 18, pp. 442-446

Kauffman, T., Brady, F.X., Puletti, P.P., and Raykovitz, G., (1992) Poly hydroxybutyrate/hydroxyvalerate based hot melt adhesive. US patent 5169889.

Khanna, S., and. Srivastava, A.K., (2005) Recent advances in microbial polyhydroxyalkanoates. Process Biochemistry, 40, pp. 607-619.

Knoerzer, A.R., and Rodgers, B.D., (2012) Environmentally-friendly multi-layer flexible film having barrier properties. US patent 8105667.

Lafferty R.M., Korsatko, B., and Korsatko, W., (1988) Microbial Production of Poly-β-hydroxybutyric Acid. in Biotechnology, H. J., Rehm, and G. Reed, Eds., pp. 135-176, VCH Publishers, New York.

Li, J., Shang, G.G., You, M.L., Peng, S.W., Wang, Z.H., Wu, H.N., Chen, G.Q., (2011) Endotoxin Removing Method Based on Lipopolysaccharide Binding Protein and Polyhydroxyalkanoate Binding Protein PhaP. Biomacromolecules, 12, pp. 602-609.

Lomas, A.J.,. Chen, G.G.Q., El-Haj A.J., and Forsyth, N.R., (2012) Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) supports adhesion and migration of mesenchymal stem cells and tenocytes. World Journal of Stem Cells, 4(9), p. 94-100.

Lu , X.Y., Ciraolo , E., Stefenia , R., Chen , G.Q., Zhang, Y., and Hirsch, E., (2011) Sustained release of PI3K inhibitor from PHA nanoparticles and in vitro growth inhibition of cancer cell lines. Applied Microbiology and Biotechnology, 89(5), p. 1423-1433.

Madison L.L., and Huisman, G.W., (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiology and Molecular Biology Reviews, 63(1), 21-53.

Malm, T., Bowald, S., Bylock, A., Busch, C., and Saldeen, T., (1994) Enlargement of the right-ventricular out flow tract and the pulmonary-artery with a new biodegradable patch in transannular position. European Surgical Research, 26, p. 298-308.

Marchessault, R.H., LePoutre, P.F., and. Wrist, P.E., (1995) Latex of poly-β-hydroxyalkanoates for treating fiber constructs and coating paper. US patent 5451456.

Matlouthi, M., (Ed). (1994) Food Packaging and Preservation. Aspen Publishers, New York, NY.

Martin, D.P., and. Williams, S F., (2003) Medical applications of poly 4-hydroxybutyrate: a strong flexible absorbable biomaterial. Biochemical Engineering Journal, 16, pp. 97-105.

Mohanna, P.N., Terenghi, G., and Wiberg, M., (2005) Composite PHB-GGF conduit for long nerve gap repair: a long-term evaluation. Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, 39, p. 129-137.

Najdegerami , E.H., Tran, T.N., Defoirdt , T., Marzorati , M., Sorgeloos , P., Boon , N., and Bossier, P., (2012) Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community,” FEMS Microbiology Ecology. 79(1), pp. 25-33.

Nhan, D.T., Wille, M., De Schryver, P., Defoirdt, T., Bossier, P., and Sorgeloos, P., (2010) The effect of poly [β]-hydroxybutyrate on larviculture of the giant freshwater prawn Macrobrachium rosenbergii. Aquaculture, 302(1-2), pp.76-81.

Noda, I., and Fairfield, O.H., (2000) Fibers, nonwoven fabrics, and absorbent articles comprising a biodegradable polyhydroxyalkanoate comprising 3-hydroxybutyrate and 3- hydroxyhexanoate. US patent 6013590.

Noda, I., Bond, E.B., and Melik, D.H., (2005) Fibers comprising polyhydroxyalkanoate copolymer/polylactic acid polymer or copolymer blends. US Patent 6905987.

Peng, Q., Zhang, Z., Gong, R.T., Chen, G.Q., and. Sun, X.A., (2012) Rapid-acting, long-acting insulin formulation based on a phospholipid complex loaded PHBHHx nanoparticles. Biomaterials, 33(5), p.1583-1588.

Peter, V., and Rehm, B.H.A., (2005) In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases. FEMS Microbiology Letters, 248, p. 93-100.

Philip, S., Keshavarz, T., and Roy, I., (2007) Polyhydroxyalkanoates: biodegradable polymers with a range of applications. Journal of Chemical Technology & Biotechnology, 82, pp. 233-247.

Pouton, C.W., and Akhtar, S., (1996) Biosynthetic polyhydroxyalkanoates and their potential in drug delivery. Advanced Drug Delivery Reviews, 18, p. 133-162.

Ren, Q., Ruth, K., Thöny-Meyer, L., and Zinn, M., (2010) Enatiomerically pure hydroxycarboxylic acids: current approaches and future perspectives. Applied Microbiology and Biotechnology, vol. 87(1), pp. 41-52.

Reusch, R.N., and Reusch, W.H., (1993) Branched polyhydroxyalkanoate polymer salt compositions and method of preparation. US patent 5266422.

Reusch, R.N., and Reusch, W.H., (1996) Branched poly (hydroxyalkanoate) polymer salt electrolytic compositions and method of preparation. US patent RE35257.

Ruth, K., Grubelnik, A., Hartmann, R., Egli, T., Zinn, M., and Ren, Q., (2007) Efficient production of (R)-3-hydroxycarboxylic acids by biotechnological conversion of polyhydroxyalkanoates and their purification. Biomacromolecules, 8, pp. 279-286.

Rutherford, R., Hammar, W.J., and Babu, G.N., (1997) Poly (β-hydroxyorganoate) pressure sensitive adhesive compositions, US patent 5614576.

Sandoval, A., Arias-Barrau, E., Bermejo, F., Canedo, L., Naharro, G., Olivera, E., and Luengo, J., (2005) Production of 3-hydroxy-n-phenylalkanoic acids by a genetically engineered strain of Pseudomonas putida. Applied Microbiology and Biotechnology, 67, pp. 97-105.

Singh, N.K., Purkayastha, B.P.D., Roy, J.K., Banik, R.M., Prasad Gonugunta, P., Misra, M., and Maiti, P., (2011) Tuned biodegradation using poly(hydroxybutyrate-co-valerate) nanobiohybrids: Emerging biomaterials for tissue engineering and drug delivery. Journal of Materials Chemistry, 21, p. 15919-15927.

Steel, M.L., and Norton-Berry, P., (1986) Non-woven fibrous material. US patent 4603070.

Sudesh, K., (2013) Potential Applications of PHA,” in Polyhydroxyalkanoates from Palm Oil: Biodegradable Plastics, pp. 79-100, Springer Briefs in Microbiology Springer Berlin Heidelberg.

Tasaki, O., Hiraide, A., Shiozaki, T., Yamamura, H., Ninomiya, N., and Sugimoto, H., (1999) The dimer and trimer of 3-hydroxybutyrate oligomer as a precursor of ketone bodies for nutritional care. Journal of Parenteral and Enteral Nutrition, 23, pp. 321-325.

Terenghi, G., Mohanna, P., and Martin, D.P., (2011) Polyhydroxyalkanoate nerve regeneration devices. US patent 2006/0287659 A1.

Tieu, K., Perier, C., Caspersen, C., Teismann, P., Wu, D.C., Yan, S.D., Naini, A., Vila, M., Jackson-Lewis, V., Ramasamy, R., and Przedborski, S., (2003) D-beta-hydroxybutyrate rescues mitochondrial respiration and mitigates features of Parkinson disease,” Journal of Clinical Investigation, 112, pp. 892-901.

Vander Walle, A.M., Busiman, G.J.H., Weusthuis, R.A, and Eggink, G., (1999) Development of environmentally friendly coatings and paints using medium-chain-length poly(3-hydroxyalkanoates) as the polymer binder. International Journal of Biological Macromolecules. 25, pp.123-128.

Van Cam, D.T., Van Hao, N., Dierckens, K., Defoirdt, T., Boon, N., Sorgeloos, P., and Bossier, P., (2009) Novel approach of using homoserine lactone-degrading and poly-[β]-hydroxybutyrate accumulating bacteria to protect Artemia from the pathogenic effects of Vibrio harveyi. Aquaculture, 291, pp. 23-30,.

Wang, S.Y., Wang, Z., Liu, M.M., Xu, Y., Zhang, X.J., and Chen, G.Q., (2010) Properties of a new gasoline oxygenate blend component: 3-hydroxybutyrate methyl ester produced from bacterial poly-3- hydroxybutyrate,” Biomass and Bioenergy, 34(8), p. 1216-1222.

Wang, Y., Bian, Y.Z., Wu, Q., and Chen, G.Q., (2008) Evaluation of three-dimensional scaffolds prepared from poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits. Biomaterials, 29, pp. 2858-2868.

Weisheng, L., Orfan, C.P., Liu, J., and. Foster, J.W., (2001) Environmentally friendly chewing gum bases including polyhydroxyalkanoates. US Patent 6194008.

Xiong, Y.C., Yao, Y.C., Zhan, X.Y., and Chen, G.Q., (2010) Application of polyhydroxyalkanoates nanoparticles as intracellular sustained drug-release vectors. Journal of Biomaterials Science, Polymer Edition, 21(1), p. 127-140.

Yagmurlu, M.F., Korkusuz, F., Gursel, I., Korkusuz, P., Ors, U., and Hasirci, V., (1999) Sulbactam-cefoperazone polyhydroxybutyrate- co-hydroxyvalerate (PHBV) local antibiotic deliverysystem: in vivo effectiveness and biocompatibility in the treatment of implant-related experimental osteomyelitis. Journal of Biomedical Materials Research, 46, p. 494-503.

Yalpani, M., (1993a) Polyhydroxyalkanoate flavor delivery system. US patent 5225227.

Yalpani, M., (1993b) Polyhydroxyalkanoate cream substitutes. US patent 5229158.

Yang, H.X., Sun, M., Zhang, Y., and Zhou, P., (2011) Degradable PHBHHx Modified by the Silk Fibroin for the Applications of Cardiovascular Tissue Engineering,” ISRN Materials Science, Vol. 2011, Article ID 389872.

Ye, C., Hu, P., Ma, M.X., Xiang, Y., Liu, R.G., and Shang, X.W., (2009) PHB/PHBHHx scaffolds and human adipose-derived stem cells for cartilage tissue engineering. Biomaterials, 30, 440-4406.

You, M., Peng, G., Li, J., Ma, P., Wang, Z., Shu, W., Peng, S., and Chen, G.Q., (2011) Chondrogenic differentiation of human bone marrow mesenchymal stem cells on polyhydroxyalkanoates (PHA) scaffolds coated with PHA granule binding protein PhaP fused with RGD Peptide. Biomaterials, 32, p. 2305-13.

Young, R.C., Wiberg, M., and Terenghi, G., (2002) Poly-3-hydroxybutyrate (PHB): a resorbable conduit for long-gap repair in peripheral nerves. British Journal of Plastic Surgery, 55, p. 235-240.

Zhang, X., Luo, R., Wang, Z., Deng, Y., and Chen, G.Q., (2009) Application of (R)-3- hydroxyalkanoate methyl esters derived from microbial polyhydroxyalkanoates as novel biofuels. Biomacromolecules, 10, p. 707-711.

Zhou, J., Peng, S.W., Wang, Y.Y., Zheng, S.B., Wang, Y., and Chen, G.Q., (2010) The use of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tarsal repair in eyelid reconstruction in the rat. Biomaterials, 31(29), p.512-7518.

Zhao, Y., Zou, B., Shi, Z.Y., Wu, Q., and Chen, G.Q. (2007) The effect of 3-hydroxybutyrate on the in vitro differentiation of murine osteoblast MC3T3-E1 and in vivo bone formation in ovariectomized rats. Biomaterials, 28(30), pp. 63-73.

Zou, X.H.,. Li, H.M., Wang, S., Leski, M., Yao, Y.C., Yang, X.D., Huang, Q.J., and Chen, G.Q., (2009) The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice. Biomaterials, 30, pp. 1532-1541.


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